Takes the guesswork out of siRNA generation
Abingdon, UK — AMSBIO has introduced a Dicer siRNA Generation Kit that mimics the natural RNA interference process by using recombinant human dicer enzyme, a double-stranded RNA-specific endonuclease, to cleave in vitro transcribed dsRNA templates into a pool of 22 bp siRNAs.
The new kit enables easy and cost-effective generation of a large number of small interfering RNAs (siRNAs) from full-length target genes.
For maximum convenience, the Dicer siRNA Generation Kit contains everything that is required for preparing double stranded RNA, RNA cleavage, siRNA cleanup and transfection.
Using the Dicer siRNA Generation Kittakes the guesswork out of siRNA generation as preparing a mixture of siRNAs has a better chance of success than a single siRNA design. Consequently labs using the kit will benefit as they waste less time and money on failed siRNA designs and are able to screen more regions of genes for silencing.
Each AMSBIODicer siRNA Generation Kit contains sufficient material for transcribing, cleaving, and transfecting siRNAs for 50 transfection experiments in 24-well plates with up to 5 different genes.
For further information please visit http://www.amsbio.com/small-interfering-RNA-siRNA.aspx or contact AMSBIO on on+44-1235-828200 (Europe / ROW) / +1-800-987-0985 (North America) or email firstname.lastname@example.org.
siRNAs are 21- to 23-nucleotide RNA molecules that can cause targeted gene silencing in mammalian cells through a process known as RNA interference. In nature, siRNAs are generated by ribonucleic III cleavage of longer double stranded RNAs (dsRNAs).
When dsRNAs are transfected directly into mammalian cells, they activate the interferon system and provoke non-specific gene suppression and cytotoxic response. siRNAs have proven to be effective at specifically silencing gene expression without causing any interferon response.
AMS Biotechnology (AMSBIO), founded in 1987, is recognized today as a leading company contributing to the acceleration of discovery through the provision of cutting-edge life science technology products and services for research and development in the medical, nutrition, cosmetics and energy industries. The AMSBIO range includes specialist antibodies, peptides and recombinant proteins. In addition the company is able to draw upon in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. Widely acknowledged as experts in cell culture, AMSBIO partners with clients in tailoring cell systems to enhance screening outcomes and eventual prognosis. With a range of molecular detection reagents, and a significant Biorepository the company can also provide tissue DNA, RNA, protein and microarray products. Key research areas for these products include: Oncology, Regenerative Medicine, Environmental Analysis, Cytotoxicity Screening, Glycomics and Stem Cell Biology.
AMS Biotechnology (AMSBIO)
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